Biocatalysts can be excellent tools for developing very competitive and durable processes. We have broad experience in use of biocatalysts, from traditional lipase enzyme catalyzed resolutions to use of highly engineered enzymes for enantioselective transformations.
When target projects present opportunities in which enzymatic reactions clearly offer a desirable option, we can scope out the viability with screening activity.
Screening is carried out in parallel for the desired transformation against different enzymes from our library.
Typically, the initial screening is run at mg level and is initially focused on reaction activity under certain conditions, and those enzymes with zero activity are removed from the test. Those with positive activity are then tested in slightly larger scale with a more realistic enzyme loading scenario.
For enantioselective processes, after activity is found the enantioselectivity is tested, and usually the results there are excellent, with the most active enzyme also giving very high enantioselectivity.
Examples of Bio-catalysts
• Imine reductase
• Amino acylase
• Baeyer Villager Mono Oxygenase
• Esterases, Lipases and Proteases
Biocatalytic process optimization
Finding an enzyme that will carry out the desired transformation is a critical first step. However, to be viable, the processes need optimization and scale-up activity just like any other process. There are some unique challenges involved in scale-up of enzymatic processes, such as the removal of the enzyme proteins or any carriers and cofactors involved.